Grow Mycelium

Table of content

Library

Tempeh bowl

Making

Evolution over the weeks

TextileLab contribution

Body Incubator

Created 14/03/2019

Updated 14/03/2019

Library

Readings

  • Radical Mycology A to Z, Peter McCoy and Natassja Noell
  • Mycelium running - how mushrooms can help save the world, Paul Stamets
  • Growing Gourmet and medicinal mushrooms, Paul Stamets
  • Fungi in bioremediation, G.-M. Gadd
  • Psilocybin - Magic mushroom grower's guide, O.T. OSS and O.N. OERIC
  • The mushroom cultivator - a practical guide to growing mushroom at home, Paul Stamets and J.S. Chilton
  • Mycoremediation - fungal bioremediation, Harbhajan Singh
  • Mycorrhizal biotechnology, D. Thangadurai, C.A. Busso, M. Hijri
  • Cordyceps Cultivation handbook, William Padilla-Brown
  • Fungi Materials Manual by Elise Elsacker (researcher), Kristel Peters (shoe designer), Winnie Poncelet (bio-engineer) from BioFab Forum
  • Mushroom cultivation manual for the small mushroom entrepreneur
  • Mycelium Material Study, Studio MUR MUR Chicago
  • Nature: Collaborations in Design, Cooper Hewitt Design Triennial co-organized with Cube design museum
  • The Mushroom at the End of the World, Anna Lowenhaupt Tsing

Videos/ Movies

Visits

Workshops

References

Suppliers and recipes

Contact

  • Conor Croasdell (former intern at Waag) → see his project a body heat incubator
  • Candyce Dryburgh (former intern at Waag and founder of local forms)
  • Inga Tilda (former intern at mediamatic)
  • Yaël (researcher at TU Delft)
  • Wasabii (researcher at TU Delft)

Created 14/03/2019

Updated 14/03/2019

Tempeh bowl

Tempeh

What is tempeh and how to make some

Why create a composite material with Mycelium

First shape try

Before the fermentation process, I put the preparation in a bowl against the edges and I put a smaller bowl above. I wrapped it up with plastic film and made holes with a knife.

As you can see on the picture, the mycelium didn't grow in the bottom of the bowl because because it didn't have enough air.

Created 20/07/2019

Updated 17/09/2020

Making

I wanted to do some growth test with mycelium so I inoculate 3 types of mycelium in 4 types of nutrient medium.

. Schyzophilum Grey Oyster Tempeh starter
Coffee Ground x x x
Dog Food x x x
Liquid Nutrient x x x
Agar Nutrient x x x

Make and sterilise the medium

Coffee Grounds

Coffee grounds are sterilised by the process of making coffee (when the boiled water pass into the coffee) so I directly took some coffee ground after making me a coffee and I spread it in 3 sterile petri dishes. Then I let them cool down to room temperature.

Dog Food

I crushed dried dog kibble and I spread them in 3 glass petri dishes that I reserved.

Liquid Nutrient

I prepared a pot with:

  • 250ml distilled water
  • 0,75g of Malt extract
  • 0,75g of Yeast extract
  • 1,25g of Peptone
  • 2,5g of Glucose

I mixed it and I reserved.

(The recipe come from the biofabforum )

Agar Nutrient

I mixed 7g of agar powder with 250ml of distilled water in a pot and I reserved.

(The recipe was on the agar powder box)

Autoclaving with pressure cooker

  1. I first put pieces of autoclave tape in each petri dishes and pots and I put all of them in the pressure cooker.
  2. I filled 1/3 of the pressure cooker with water
  3. I closed the autoclave (make sure it's perfectly closed), lock it and turn on the heat to the maximum
  4. It takes normally 10-20 min for the red pressure valve goes up (it's like a little button located on the lid of the autoclave)
  5. Then I turned the heat to the middle point and wait 15-20 min more
  6. I Released the pressure by keeping the valve up with a tweezers and then I opened the If there is black lines on the autoclave tape, the sterilization process is done !

Then, I let everything cool down to room temperature before the inoculation.

Inoculation

See below my inoculation set up:

To create a sterilised area, I turn on the gaz burner and spread some ethanol around it. Ethanol evaporates and this creates a sterile area between the table and the fire inside where you can work.

I filled all the petri dishes with the different kind of medium.

I first inoculated mycelium schizophilum in the 4 different kind of nutrients.

I then inoculated mycelium grey oyster in the 4 different kind of nutrients.

I finally inoculated tempeh starter in the 4 different kind of nutrients.

And I let them incubate at 26°C, and wait and see.

Created 09/10/2019

Updated 09/10/2019

TextileLab contribution

Find below my contribution to the TextileLab biomaterial library of Waag:

Mycelium Leather

→ get the .pdf file

Test 1

For steps 1-2-3, read my post Grow Mycelium - making to know how to inoculate some mycelium into the liquid nutrient medium.

the result after 10 days of incubation (step 4)
the result after 10 days of incubation (step 4)
the result after 10 days of incubation (step 4)
the result after 10 days of incubation (step 4)
Harvest the mycelium sheet (step 5)
Harvest the mycelium sheet (step 5)
Plasticise the mycelium and let it soak for several hours (step 6)
Plasticise the mycelium and let it soak for several hours (step 6)
the result of the dried mycelium sheet after several hours in the oven (step 7)
the result of the dried mycelium sheet after several hours in the oven (step 7)
the result of the dried mycelium sheet after several hours in the oven (step 7)
the result of the dried mycelium sheet after several hours in the oven (step 7)

Test 2

The result after 10 days of incubation
The result after 10 days of incubation
The result after 20 days of incubation. I wanted to wait a bit more and hoped to have it more dense but it did the opposite, it started to reduce. I guess not enough food anymore and so it slowly started to die
The result after 20 days of incubation. I wanted to wait a bit more and hoped to have it more dense but it did the opposite, it started to reduce. I guess not enough food anymore and so it slowly started to die
Anyway, I still plasticised and dried the mycelium sheet and the result is still fine but very thin
Anyway, I still plasticised and dried the mycelium sheet and the result is still fine but very thin
I also did a try with some mycelium inoculated in dog food because the medium and the mycelium were really compacted together and the result is interesting because it is thicker than the other tests
I also did a try with some mycelium inoculated in dog food because the medium and the mycelium were really compacted together and the result is interesting because it is thicker than the other tests

Mycelium and Straw

→ get the .pdf file

Read my project Mycelium, straw and weaving to see the process.

Created 01/11/2019

Updated 01/11/2019

Body Incubator

Wear this necklace for 3 weeks and incubate edible mushrooms with the heat of your body. If you take well care of your micro pet, you may harvest some!!

The necklace

  • I bought small petri dishes in Pidiscat.cat
  • I designed and 3D printed a petri dish holder
  • I knotted a rope to go around the neck
Using my Prusa MINI printer
Using my Prusa MINI printer
I tried to be more efficient by printing 4 at the same time but I had some bed adhesion issue
I tried to be more efficient by printing 4 at the same time but I had some bed adhesion issue

Inoculation

I inoculated some grey oyster spawn in coffee ground. How to inoculate mycelium

Friends

I offered a few necklaces to my friends for the experience and the results were fruitful.

Incubation period

Sally
Sally
Me
Me
Benny
Benny
Parents
Parents
Melina
Melina
Jess
Jess
Lilas
Lilas
Me
Me
Me
Me
Benny
Benny
Benny
Benny
Vera
Vera
Melina
Melina

Fruiting periode

me
me
Benny
Benny
Melina
Melina
Lilas
Lilas
Vera
Vera
Melina
Melina
Parents (it's a joke!)
Parents (it's a joke!)
Melina (it isn't a joke!)
Melina (it isn't a joke!)

Created 14/12/2020

Updated 12/02/2021